ALiCE® Cell-Free Protein Expression Midi Kit (24 reactions)

Features

• Produces up to 3 mg/mL protein in under 48 hours.
• Simple reaction set-up – just add DNA
• Capable of catalyzing complex post-translational modifications

Description

The ALiCE cell-free protein expression kits sets a new benchmark for protein production. The kits contain a lysate derived from N. tabacum c.v. BY-2 cells which has been specially optimized to produce consistently high protein yields of up to 3 mg per mL (eYFP). Inclusion of native microsomes derived from endoplasmic reticulum and Golgi apparatus means the lysate is also capable of producing proteins with complex eukaryotic post-translational modifications, including di-sulfide bonds and glycosylation. The kits are provided with two vectors, pALiCE01 and pALiCE02 respectively, which enable the user to produce proteins via cytosolic or microsomal pathways. These elements combined, provide a powerful expression tool capable of producing even the most difficult-to-express proteins.

The kits are available in three sizes to adapt to your protein expression needs.

Performance

Every batch of the ALiCE lysate undergoes rigorous quality control testing. Before release, the lysate must meet our stringent criteria of achieving 3 mg/mL eYFP following a 48 hour reaction period.

Usage

The ALiCE kits were developed specifically for ease of use. Expression of your protein of interest follows a simple four-step procedure:

1. Clone your gene of interest into the ALiCE vector of your choosing
2. Add your plasmid DNA to a thawed vial of ALiCE lysate
3. Incubate at 25°C with aeration for 24-48 hours.
4. Collect your protein

For the full details of the procedure, download our user manual.

The ALiCE Cell-free protein expression kits are intended for research use only.

Storage conditions

The ALiCE Cell-Free Protein Expression Kits are delivered on dry ice. They should be transferred directly to a -80°C freezer upon delivery.